国产在线综合网站,婷色综合av,精品美女久久久久久高潮,嫩草伊人久久精品少妇av

撥號(hào)18861759551

你的位置:首頁(yè) > 技術(shù)文章 > 顯微鏡和三色染色鏡的分析

技術(shù)文章

顯微鏡和三色染色鏡的分析

技術(shù)文章

Microscopy and the Analysis of a Trichrome Stain

When imaging biological material, more often than not it is extremely difficult to differentiate between various organelles and tissues. Light scatters differently from each structure, but the change in contrast is so slight it becomes a strain to analyze the specimen. The first triple stain used to increase contrast and improve recognition dates back to 1880. One of the early methods of staining tissues for histology was developed by Claude Pierre Masson, and has since been coined the Masson trichrome stain.

 

Masson's trichrome stain is incredibly effective in differentiating cells and their components from the surrounding connective tissues. One of the most common stain types, which has been used on the dermal tissue sample seen in the images within this article, yields a number of colors where cell nuclei appear dark red, collagen and other tissues appear green or blue, and cell cylasm appear red/purple (Jones, 2010). These stains have been imaged under brightfield and darkfield illumination, and then again with specific filters to selectively focus on the cellular constituents of the epidermis. The primary application for the epidermal trichrome stains is differentiating healthy collagen and muscles from connective tissues onset with tumorigenesis. Typically the tumors proliferate from muscle cells and fibroblasts deep in the dermal tissue (Blitterswijk, 2010).

 

List of Components for Analysis of Trichrome Stain Setup

 

Description

Stock No.

1.

20X Mitutoyo Plan Apo Infinity Corrected Long WD Objective

#46-145

2.

MT-1 Accessory Tube Lens

#54-774

3.

TECHSPEC® Mitutoyo MT-1/MT-2 C-mount Adapter

#58-329

4.

543nm CWL, 22nm Bandwidth, OD 6 Fluorescence Filter

#67-032

5.

605nm CWL, 15nm Bandwidth, OD 6 Fluorescence Filter

#86-356

6.

EO-3112C ½" CMOS Color USB Camera

#59-367

7.

115V, MI-150 Fiber Optic Illuminator

#59-235

8.

4.25" x 3.37" Fiber Optic Backlight

#39-826

 

The image setup consists of a number of components, which are differentiated as optical and imaging components. The imaging products that will be discussed are the camera and illumination, and the optical components that will be discussed include the microscope objective lens and optical filters.

Figure 1: Brightfield Image of Dermal Tissue

 

Figure 2: Darkfield Imaging of Dermal Tissue

 

When comparing Figures 1 and 2, the visual differences are significant. A brightfield image is formed with the illumination source below the sample, and then transmitted light propagates through the sample to the sensor forming a bright, white background with sharp color. A darkfield image is formed by directing light at an oblique angle through the sample, forming a hollow cone of light which is collected by the objective. Darkfield illumination typically yields a dark background with sharp color, but in the case of Figure 2, the collagen and muscle fibers interfered with the light path and caused a blur of light and color. The dark background is hardly evident and only two distinct colors are visible. When analyzing histological stains, brightfield illumination is the preferred technique for lighting a sample.

Figure 3: Brightfield Image of Dermal Tissue filtered with Green

Figure 4: Brightfield Image of Dermal Tissue filtered with Red

 

When comparing Figure 3 with Figure 4, there is once again a significant visual difference. The most obvious feature is the change in color from green to red due to a different hardcoated filter being positioned in the optical path. The less obvious difference is the varying contrast levels caused by the filters at specific regions of the dermal tissue. For example, Figure 3 exhibits a distinct ring at the central region of the cell with additional matter within. In Figure 4, the ring is extremely faint and the internal matter is not visible. With that said, the cell and surrounding dense materials are more evident in Figure 3, whereas the muscle fibers and collagen are more pronounced in Figure 4.

 

Researchers have discovered a number of methods to quickly and accuray diagnose many ailments, such as many forms of cancer. As technologies continue to advance at an increasing rate, the cost of histology analysis will continue to decrease as images and videos can be easily transmitted across the globe. Even with constantly changing technology, the trichrome stain is still one of the most powerful techniques available in the field of histology and diagnostics over 100 years later.

 

Microscopy and the Analysis of a Trichrome Stain

When imaging biological material, more often than not it is extremely difficult to differentiate between various organelles and tissues. Light scatters differently from each structure, but the change in contrast is so slight it becomes a strain to analyze the specimen. The first triple stain used to increase contrast and improve recognition dates back to 1880. One of the early methods of staining tissues for histology was developed by Claude Pierre Masson, and has since been coined the Masson trichrome stain.

 

Masson's trichrome stain is incredibly effective in differentiating cells and their components from the surrounding connective tissues. One of the most common stain types, which has been used on the dermal tissue sample seen in the images within this article, yields a number of colors where cell nuclei appear dark red, collagen and other tissues appear green or blue, and cell cylasm appear red/purple (Jones, 2010). These stains have been imaged under brightfield and darkfield illumination, and then again with specific filters to selectively focus on the cellular constituents of the epidermis. The primary application for the epidermal trichrome stains is differentiating healthy collagen and muscles from connective tissues onset with tumorigenesis. Typically the tumors proliferate from muscle cells and fibroblasts deep in the dermal tissue (Blitterswijk, 2010).

 

List of Components for Analysis of Trichrome Stain Setup

 

Description

Stock No.

1.

20X Mitutoyo Plan Apo Infinity Corrected Long WD Objective

#46-145

2.

MT-1 Accessory Tube Lens

#54-774

3.

TECHSPEC® Mitutoyo MT-1/MT-2 C-mount Adapter

#58-329

4.

543nm CWL, 22nm Bandwidth, OD 6 Fluorescence Filter

#67-032

5.

605nm CWL, 15nm Bandwidth, OD 6 Fluorescence Filter

#86-356

6.

EO-3112C ½" CMOS Color USB Camera

#59-367

7.

115V, MI-150 Fiber Optic Illuminator

#59-235

8.

4.25" x 3.37" Fiber Optic Backlight

#39-826

 

The image setup consists of a number of components, which are differentiated as optical and imaging components. The imaging products that will be discussed are the camera and illumination, and the optical components that will be discussed include the microscope objective lens and optical filters.

Figure 1: Brightfield Image of Dermal Tissue

 

Figure 2: Darkfield Imaging of Dermal Tissue

 

When comparing Figures 1 and 2, the visual differences are significant. A brightfield image is formed with the illumination source below the sample, and then transmitted light propagates through the sample to the sensor forming a bright, white background with sharp color. A darkfield image is formed by directing light at an oblique angle through the sample, forming a hollow cone of light which is collected by the objective. Darkfield illumination typically yields a dark background with sharp color, but in the case of Figure 2, the collagen and muscle fibers interfered with the light path and caused a blur of light and color. The dark background is hardly evident and only two distinct colors are visible. When analyzing histological stains, brightfield illumination is the preferred technique for lighting a sample.

Figure 3: Brightfield Image of Dermal Tissue filtered with Green

Figure 4: Brightfield Image of Dermal Tissue filtered with Red

 

When comparing Figure 3 with Figure 4, there is once again a significant visual difference. The most obvious feature is the change in color from green to red due to a different hardcoated filter being positioned in the optical path. The less obvious difference is the varying contrast levels caused by the filters at specific regions of the dermal tissue. For example, Figure 3 exhibits a distinct ring at the central region of the cell with additional matter within. In Figure 4, the ring is extremely faint and the internal matter is not visible. With that said, the cell and surrounding dense materials are more evident in Figure 3, whereas the muscle fibers and collagen are more pronounced in Figure 4.

 

Researchers have discovered a number of methods to quickly and accuray diagnose many ailments, such as many forms of cancer. As technologies continue to advance at an increasing rate, the cost of histology analysis will continue to decrease as images and videos can be easily transmitted across the globe. Even with constantly changing technology, the trichrome stain is still one of the most powerful techniques available in the field of histology and diagnostics over 100 years later.

聯(lián)系我們

地址:江蘇省江陰市人民東路1091號(hào)1017室 傳真:0510-68836817 Email:sales@rympo.com
24小時(shí)在線客服,為您服務(wù)!

版權(quán)所有 © 2025 江陰韻翔光電技術(shù)有限公司 備案號(hào):蘇ICP備16003332號(hào)-1 技術(shù)支持:化工儀器網(wǎng) 管理登陸 GoogleSitemap

在線咨詢
QQ客服
QQ:17041053
電話咨詢
0510-68836815
關(guān)注微信
久久www兔费人成| 日日夜夜硬硬爽爽| 外国大鸡巴搞h| 日韩青青操tt视频| 色婷婷1区2区3区在线观看| 欧美高清1区| 久久久少妇蜜臀| 亚洲日韩香蕉网一区| 吉林省| 国产精品酒店AV久久久| 午夜黄色电影大全| 人妻在线九色| 国产精品一区不卡av| 欧美一级乳片| 91熟女天天久久另类视频| 日本播放器免费在线观看| 波多野结衣欧美| 成人五月天婷婷天| 色域一区二区| 日本午夜成人在线| 欧美精品午夜| 无码区一二| 屁股网址久久爱| 鲁鲁鲁A片| 巴南区| 国产精品免费不卡在线| 国产永久久久久久久女同| 国产成人毛片精品不卡在| 国产三级不卡一二三四区| 黄片污视频免费在线观看| 亚洲丝袜脚交乱伦| 黄色香蕉视频网站一区| 久久精品 日韩高清| 无码精品视频一二区| 伦一区二区三区| 日本成人伦理午夜福利 | 欧美日韩亚| 欧美亚洲日韩激情一区| 欧美日逼一二三四| 国产V日韩V| 日韩欧美产精品免费|